7LW9
Human Exonuclease 5 crystal structure in complex with ssDNA, Sm, and Na
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ALS BEAMLINE 8.3.1 |
| Synchrotron site | ALS |
| Beamline | 8.3.1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2019-04-04 |
| Detector | DECTRIS PILATUS3 6M |
| Wavelength(s) | 1.1161 |
| Spacegroup name | P 2 21 21 |
| Unit cell lengths | 51.073, 83.664, 96.150 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 45.105 - 2.710 |
| R-factor | 0.1967 |
| Rwork | 0.194 |
| R-free | 0.25320 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 7lw7 |
| RMSD bond length | 0.003 |
| RMSD bond angle | 0.590 |
| Data reduction software | XDS |
| Data scaling software | Aimless (0.7.4) |
| Phasing software | PHASER (2.7.16) |
| Refinement software | PHENIX (1.16_3549) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 45.105 | 45.100 | 2.840 |
| High resolution limit [Å] | 2.710 | 8.990 | 2.710 |
| Rmerge | 0.317 | 0.057 | 3.070 |
| Rmeas | 0.329 | 0.060 | 3.194 |
| Rpim | 0.090 | 0.018 | 0.870 |
| Total number of observations | 4028 | 19957 | |
| Number of reflections | 11691 | 380 | 1516 |
| <I/σ(I)> | 8.9 | 35.1 | 1.1 |
| Completeness [%] | 99.7 | 99.2 | 99.7 |
| Redundancy | 13 | 10.6 | 13.2 |
| CC(1/2) | 0.995 | 0.998 | 0.394 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 293 | 20 % PEG 3350 and 0.2 M ammonium acetate |
