7LK4
Crystal structure of BAK L100A in complex with activating antibody fragments
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | AUSTRALIAN SYNCHROTRON BEAMLINE MX1 |
| Synchrotron site | Australian Synchrotron |
| Beamline | MX1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2019-02-28 |
| Detector | DECTRIS EIGER2 X 9M |
| Wavelength(s) | 0.9537 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 57.170, 271.800, 74.040 |
| Unit cell angles | 90.00, 91.79, 90.00 |
Refinement procedure
| Resolution | 39.385 - 3.100 |
| R-factor | 0.2164 |
| Rwork | 0.214 |
| R-free | 0.26210 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 5w5x |
| RMSD bond length | 0.002 |
| RMSD bond angle | 0.502 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.13_2998) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 39.385 | 3.210 |
| High resolution limit [Å] | 3.100 | 3.100 |
| Number of reflections | 40131 | 3207 |
| <I/σ(I)> | 6.8 | 1.2 |
| Completeness [%] | 98.5 | 98.1 |
| Redundancy | 3.5 | |
| CC(1/2) | 0.980 | 0.430 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 281 | 38.9 % 2-methyl-2,4-pentanediol, 0.1 M Sodium cacodylate pH 6.44, 4 % Polyethylene glycol 8000 |
