7LH5
Crystal structure of the Thermus thermophilus 70S ribosome in complex with plazomicin, mRNA and tRNAs
This is a non-PDB format compatible entry.
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | CLSI BEAMLINE 08ID-1 |
| Synchrotron site | CLSI |
| Beamline | 08ID-1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2019-08-14 |
| Detector | DECTRIS PILATUS3 6M |
| Wavelength(s) | 0.97857 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 209.450, 449.379, 619.589 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 147.060 - 3.270 |
| R-factor | 0.2171 |
| Rwork | 0.214 |
| R-free | 0.27670 |
| Structure solution method | FOURIER SYNTHESIS |
| Starting model (for MR) | 4v51 |
| RMSD bond length | 0.012 |
| RMSD bond angle | 1.815 |
| Data reduction software | DIALS |
| Data scaling software | xia2 |
| Phasing software | PHENIX |
| Refinement software | PHENIX (1.16_3549) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 147.060 | 3.385 |
| High resolution limit [Å] | 3.268 | 3.268 |
| Number of reflections | 890586 | 86465 |
| <I/σ(I)> | 5.94 | |
| Completeness [%] | 99.7 | 97.48 |
| Redundancy | 6.5 | 5.4 |
| CC(1/2) | 0.986 | 0.462 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 294.15 | 100 mM TRIS-HCl, pH 7.6, 3-3.2% (w/v) PEG 20K, 7-12% (v/v) MPD, 100-200 mM arginine, 0.5 mM Beta-mercaptoethanol |
