7LH5

Crystal structure of the Thermus thermophilus 70S ribosome in complex with plazomicin, mRNA and tRNAs

This is a non-PDB format compatible entry.

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, HB (AA, CA)	16S ribosomal RNA	polymer	1520	493346.9	2	GenBank (55771382)	Thermus thermophilus HB8
2	B, IB (AB, CB)	30S ribosomal protein S2	polymer	256	29317.7	2	UniProt (P80371) Pfam (PF00318)	Thermus thermophilus (strain HB8 / ATCC 27634 / DSM 579)
3	C, JB (AC, CC)	30S ribosomal protein S3	polymer	239	26751.1	2	UniProt (P80372) Pfam (PF07650) Pfam (PF00189)	Thermus thermophilus (strain HB8 / ATCC 27634 / DSM 579)
4	D, KB (AD, CD)	30S ribosomal protein S4	polymer	209	24373.4	2	UniProt (P80373) Pfam (PF00163) Pfam (PF01479)	Thermus thermophilus (strain HB8 / ATCC 27634 / DSM 579)
5	E, LB (AE, CE)	30S ribosomal protein S5	polymer	162	17583.4	2	UniProt (Q5SHQ5) Pfam (PF00333) Pfam (PF03719)	Thermus thermophilus (strain HB8 / ATCC 27634 / DSM 579)
6	F, MB (AF, CF)	30S ribosomal protein S6	polymer	101	11988.8	2	UniProt (Q5SLP8) Pfam (PF01250)	Thermus thermophilus (strain HB8 / ATCC 27634 / DSM 579)	TS9
7	G, NB (AG, CG)	30S ribosomal protein S7	polymer	156	18051.0	2	UniProt (P17291) Pfam (PF00177)	Thermus thermophilus (strain HB8 / ATCC 27634 / DSM 579)
8	H, OB (AH, CH)	30S ribosomal protein S8	polymer	138	15868.6	2	UniProt (P0DOY9) Pfam (PF00410)	Thermus thermophilus (strain HB8 / ATCC 27634 / DSM 579)
9	I, PB (AI, CI)	30S ribosomal protein S9	polymer	128	14410.6	2	UniProt (P80374) Pfam (PF00380)	Thermus thermophilus (strain HB8 / ATCC 27634 / DSM 579)
10	J, QB (AJ, CJ)	30S ribosomal protein S10	polymer	105	11955.0	2	UniProt (Q5SHN7) Pfam (PF00338)	Thermus thermophilus (strain HB8 / ATCC 27634 / DSM 579)
11	K, RB (AK, CK)	30S ribosomal protein S11	polymer	129	13737.9	2	UniProt (P80376) Pfam (PF00411)	Thermus thermophilus (strain HB8 / ATCC 27634 / DSM 579)
12	L, SB (AL, CL)	30S ribosomal protein S12	polymer	132	14637.4	2	UniProt (Q5SHN3) Pfam (PF00164)	Thermus thermophilus (strain HB8 / ATCC 27634 / DSM 579)
13	M, TB (AM, CM)	30S ribosomal protein S13	polymer	126	14338.9	2	UniProt (P80377) Pfam (PF00416)	Thermus thermophilus (strain HB8 / ATCC 27634 / DSM 579)
14	N, UB (AN, CN)	30S ribosomal protein S14 type Z	polymer	61	7158.7	2	UniProt (P0DOY6) Pfam (PF00253)	Thermus thermophilus (strain HB8 / ATCC 27634 / DSM 579)
15	O, VB (AO, CO)	30S ribosomal protein S15	polymer	89	10578.4	2	UniProt (Q5SJ76) Pfam (PF00312)	Thermus thermophilus (strain HB8 / ATCC 27634 / DSM 579)
16	P, WB (AP, CP)	30S ribosomal protein S16	polymer	88	10410.0	2	UniProt (Q5SJH3) Pfam (PF00886)	Thermus thermophilus (strain HB8 / ATCC 27634 / DSM 579)
17	Q, XB (AQ, CQ)	30S ribosomal protein S17	polymer	105	12325.7	2	UniProt (P0DOY7) Pfam (PF00366)	Thermus thermophilus (strain HB8 / ATCC 27634 / DSM 579)
18	R, YB (AR, CR)	30S ribosomal protein S18	polymer	88	10258.3	2	UniProt (Q5SLQ0) Pfam (PF01084)	Thermus thermophilus (strain HB8 / ATCC 27634 / DSM 579)
19	S, ZB (AS, CS)	30S ribosomal protein S19	polymer	93	10605.5	2	UniProt (Q5SHP2) Pfam (PF00203)	Thermus thermophilus (strain HB8 / ATCC 27634 / DSM 579)
20	AC, T (AT, CT)	30S ribosomal protein S20	polymer	106	11736.1	2	UniProt (P80380) Pfam (PF01649)	Thermus thermophilus (strain HB8 / ATCC 27634 / DSM 579)
21	BC, U (AU, CU)	30S ribosomal protein Thx	polymer	27	3350.0	2	UniProt (Q5SIH3) Pfam (PF17070)	Thermus thermophilus (strain HB8 / ATCC 27634 / DSM 579)	S31
22	CC, V (AV, CV)	P-site tRNA fMet	polymer	77	24786.8	2	GenBank (817573384)	Escherichia coli
23	DC, FC, W, Y (AW, AY, CW, CY)	E-Site tRNA Phe and A-site tRNA Phe	polymer	76	24485.5	4	GenBank (1315725308)	Escherichia coli
24	EC, X (AX, CX)	mRNA	polymer	24	7804.7	2		Escherichia coli
25	GC, Z (B0, D0)	50S ribosomal protein L27	polymer	85	9529.1	2	UniProt (P60493) Pfam (PF01016)	Thermus thermophilus (strain HB8 / ATCC 27634 / DSM 579)
26	AA, HC (B1, D1)	50S ribosomal protein L28	polymer	98	11004.2	2	UniProt (P60494) Pfam (PF00830)	Thermus thermophilus (strain HB8 / ATCC 27634 / DSM 579)
27	BA, IC (B2, D2)	50S ribosomal protein L29	polymer	72	8670.3	2	UniProt (Q5SHP6) Pfam (PF00831)	Thermus thermophilus (strain HB8 / ATCC 27634 / DSM 579)
28	CA, JC (B3, D3)	50S ribosomal protein L30	polymer	60	6799.1	2	UniProt (Q5SHQ6) Pfam (PF00327)	Thermus thermophilus (strain HB8 / ATCC 27634 / DSM 579)
29	DA, KC (B4, D4)	50S ribosomal protein L31	polymer	71	8300.5	2	UniProt (Q5SJE1) Pfam (PF01197)	Thermus thermophilus (strain HB8 / ATCC 27634 / DSM 579)
30	EA, LC (B5, D5)	50S ribosomal protein L32	polymer	60	6722.1	2	UniProt (P80339) Pfam (PF01783)	Thermus thermophilus (strain HB8 / ATCC 27634 / DSM 579)
31	FA, MC (B6, D6)	50S ribosomal protein L33	polymer	54	6632.9	2	UniProt (P35871) Pfam (PF00471)	Thermus thermophilus (strain HB8 / ATCC 27634 / DSM 579)
32	GA, NC (B7, D7)	50S ribosomal protein L34	polymer	49	6132.4	2	UniProt (P80340) Pfam (PF00468)	Thermus thermophilus (strain HB8 / ATCC 27634 / DSM 579)
33	HA, OC (B8, D8)	50S ribosomal protein L35	polymer	65	7506.2	2	UniProt (Q5SKU1) Pfam (PF01632)	Thermus thermophilus (strain HB8 / ATCC 27634 / DSM 579)
34	IA, PC (B9, D9)	50S ribosomal protein L36	polymer	37	4435.4	2	UniProt (Q5SHR2) Pfam (PF00444)	Thermus thermophilus (strain HB8 / ATCC 27634 / DSM 579)	Ribosomal protein B
35	JA, QC (BA, DA)	23S ribosomal RNA	polymer	2839	923267.6	2		Thermus thermophilus HB8
36	KA, RC (BB, DB)	5S ribosomal RNA	polymer	122	39494.5	2	GenBank (37223181)	Thermus thermophilus HB8
37	LA, SC (BC, DC)	50S ribosomal protein L1	polymer	229	24872.7	2	UniProt (Q5SLP7) Pfam (PF00687)	Thermus thermophilus (strain HB8 / ATCC 27634 / DSM 579)
38	MA, TC (BD, DD)	50S ribosomal protein L2	polymer	276	30532.6	2	UniProt (P60405) Pfam (PF00181) Pfam (PF03947)	Thermus thermophilus (strain HB8 / ATCC 27634 / DSM 579)
39	NA, UC (BE, DE)	50S ribosomal protein L3	polymer	206	22450.2	2	UniProt (Q5SHN8) Pfam (PF00297)	Thermus thermophilus (strain HB8 / ATCC 27634 / DSM 579)
40	OA, VC (BF, DF)	50S ribosomal protein L4	polymer	210	23271.9	2	UniProt (Q5SHN9) Pfam (PF00573)	Thermus thermophilus (strain HB8 / ATCC 27634 / DSM 579)	L1e
41	PA, WC (BG, DG)	50S ribosomal protein L5	polymer	182	21061.6	2	UniProt (Q5SHQ0) Pfam (PF00281) Pfam (PF00673)	Thermus thermophilus (strain HB8 / ATCC 27634 / DSM 579)
42	QA, XC (BH, DH)	50S ribosomal protein L6	polymer	180	19568.9	2	UniProt (P0DOY8) Pfam (PF00347)	Thermus thermophilus (strain HB8 / ATCC 27634 / DSM 579)
43	RA, YC (BI, DI)	50S ribosomal protein L9	polymer	148	16422.2	2	UniProt (Q5SLQ1) Pfam (PF01281) Pfam (PF03948)	Thermus thermophilus (strain HB8 / ATCC 27634 / DSM 579)
44	SA, ZC (BJ, DJ)	50S ribosomal protein L10	polymer	173	14741.1	2		Thermus thermophilus HB8
45	AD, TA (BK, DK)	50S ribosomal protein L11	polymer	147	12528.4	2		Thermus thermophilus HB8
46	BD, UA (BN, DN)	50S ribosomal protein L13	polymer	140	15927.9	2	UniProt (P60488) Pfam (PF00572)	Thermus thermophilus (strain HB8 / ATCC 27634 / DSM 579)
47	CD, VA (BO, DO)	50S ribosomal protein L14	polymer	122	13323.6	2	UniProt (Q5SHP8) Pfam (PF00238)	Thermus thermophilus (strain HB8 / ATCC 27634 / DSM 579)
48	DD, WA (BP, DP)	50S ribosomal protein L15	polymer	150	16319.1	2	UniProt (Q5SHQ7) Pfam (PF00828)	Thermus thermophilus (strain HB8 / ATCC 27634 / DSM 579)
49	ED, XA (BQ, DQ)	50S ribosomal protein L16	polymer	141	15993.9	2	UniProt (P60489) Pfam (PF00252)	Thermus thermophilus (strain HB8 / ATCC 27634 / DSM 579)
50	FD, YA (BR, DR)	50S ribosomal protein L17	polymer	118	13750.1	2	UniProt (Q9Z9H5) Pfam (PF01196)	Thermus thermophilus (strain HB8 / ATCC 27634 / DSM 579)
51	GD, ZA (BS, DS)	50S ribosomal protein L18	polymer	112	12639.8	2	UniProt (Q5SHQ4) Pfam (PF00861)	Thermus thermophilus (strain HB8 / ATCC 27634 / DSM 579)	TL24
52	AB, HD (BT, DT)	50S ribosomal protein L19	polymer	146	17188.8	2	UniProt (P60490) Pfam (PF01245)	Thermus thermophilus (strain HB8 / ATCC 27634 / DSM 579)
53	BB, ID (BU, DU)	50S ribosomal protein L20	polymer	118	13779.3	2	UniProt (P60491) Pfam (PF00453)	Thermus thermophilus (strain HB8 / ATCC 27634 / DSM 579)
54	CB, JD (BV, DV)	50S ribosomal protein L21	polymer	101	11069.2	2	UniProt (P60492) Pfam (PF00829)	Thermus thermophilus (strain HB8 / ATCC 27634 / DSM 579)
55	DB, KD (BW, DW)	50S ribosomal protein L22	polymer	113	12808.1	2	UniProt (Q5SHP3) Pfam (PF00237)	Thermus thermophilus (strain HB8 / ATCC 27634 / DSM 579)
56	EB, LD (BX, DX)	50S ribosomal protein L23	polymer	96	10759.8	2	UniProt (Q5SHP0) Pfam (PF00276)	Thermus thermophilus (strain HB8 / ATCC 27634 / DSM 579)
57	FB, MD (BY, DY)	50S ribosomal protein L24	polymer	110	12085.6	2	UniProt (Q5SHP9) Pfam (PF00467) Pfam (PF17136)	Thermus thermophilus (strain HB8 / ATCC 27634 / DSM 579)
58	GB, ND (BZ, DZ)	50S ribosomal protein L25	polymer	206	23238.6	2	UniProt (Q5SHZ1) Pfam (PF01386) Pfam (PF14693)	Thermus thermophilus (strain HB8 / ATCC 27634 / DSM 579)	TL5
59	AAA, AAB, ABA, ABB, ACA... (BA, DA, AA, BB, BU...)	MAGNESIUM ION	non-polymer		24.3	1376	Chemie (MG)
60	KL, LNA (AA, CA)	(2S)-4-amino-N-[(1R,2S,3S,4R,5S)-5-amino-4-{[(2S,3R)-3-amino-6-{[(2-hydroxyethyl)amino]methyl}-3,4-dihydro-2H-pyran-2-y l]oxy}-2-{[3-deoxy-4-C-methyl-3-(methylamino)-beta-L-arabinopyranosyl]oxy}-3-hydroxycyclohexyl]-2-hydroxybutanamide	non-polymer		592.7	2	Chemie (EDS)
61	AOA, JN, SL, VL, VNA... (CN, B9, AD, AN, CD...)	ZINC ION	non-polymer		65.4	6	Chemie (ZN)
62	UEB (AA)	water	water		18.0	2	Chemie (HOH)

Contents of the asymmetric unit

Polymers	Number of chains	118
	Total formula weight	4554347.5
Non-Polymers*	Number of molecules	1384
	Total formula weight	35021.5
All*	Total formula weight	4589369.0

*Water molecules are not included.