7KPG
Blocking Fab 25 anti-SIRP-alpha antibody in complex with SIRP-alpha Variant 1
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | RIGAKU FR-E SUPERBRIGHT |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2019-03-26 |
Detector | RIGAKU SATURN 92 |
Wavelength(s) | 1.54 |
Spacegroup name | P 61 2 2 |
Unit cell lengths | 189.160, 189.160, 78.400 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 61.920 - 2.270 |
R-factor | 0.2367 |
Rwork | 0.234 |
R-free | 0.28190 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 2uv3 |
RMSD bond length | 0.010 |
RMSD bond angle | 1.481 |
Data reduction software | XDS |
Data scaling software | Aimless |
Phasing software | PHASER |
Refinement software | REFMAC (5.8.0230) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 61.920 | 2.320 |
High resolution limit [Å] | 2.270 | 2.270 |
Number of reflections | 38395 | 2803 |
<I/σ(I)> | 10.9 | |
Completeness [%] | 99.6 | |
Redundancy | 13.5 | |
CC(1/2) | 1.000 | 1.000 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION | 277 | 28% w/v PEG 8000, 0.1 M citric acid pH 3.5 |