7KMT

Structure of the yeast TRAPPIII-Ypt1(Rab1) complex

EM specimen

Vitrification	Yes
Instrument	FEI VITROBOT MARK IV

EM Data Collection

Microscope	FEI TALOS ARCTICA,FEI TITAN KRIOS
Electron Gun	FIELD EMISSION GUN
Accelerating Voltage (kV)	200,300
Energy Filter	GIF Bioquantum,GIF Quantum LS
Detector	GATAN K3 BIOQUANTUM (6k x 4k),FEI FALCON III (4k x 4k),GATAN K2 SUMMIT (4k x 4k)

3D Reconstruction

Method	SINGLE PARTICLE
Resolution (Å)	3.7
Software	RELION,EPU,SerialEM,PHENIX,Coot,ISOLDE,UCSF Chimera
CTF correction	PHASE FLIPPING AND AMPLITUDE CORRECTION