7KMT
Structure of the yeast TRAPPIII-Ypt1(Rab1) complex
EM specimen
Vitrification | Yes |
Instrument | FEI VITROBOT MARK IV |
EM Data Collection
Microscope | FEI TALOS ARCTICA,FEI TITAN KRIOS |
Electron Gun | FIELD EMISSION GUN |
Accelerating Voltage (kV) | 200,300 |
Energy Filter | GIF Bioquantum,GIF Quantum LS |
Detector | GATAN K3 BIOQUANTUM (6k x 4k),FEI FALCON III (4k x 4k),GATAN K2 SUMMIT (4k x 4k) |
3D Reconstruction
Method | SINGLE PARTICLE |
Resolution (Å) | 3.7 |
Software | RELION,EPU,SerialEM,PHENIX,Coot,ISOLDE,UCSF Chimera |
CTF correction | PHASE FLIPPING AND AMPLITUDE CORRECTION |