7KIU

Structure of recombinant human DNase1L3 in complex with Mg2+

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRL BEAMLINE BL14-1
Synchrotron site	SSRL
Beamline	BL14-1
Temperature [K]	100
Detector technology	CCD
Collection date	2019-12-15
Detector	RAYONIX MX325HE
Wavelength(s)	1.12709
Spacegroup name	P 1
Unit cell lengths	52.313, 72.671, 73.169
Unit cell angles	85.39, 80.56, 88.39

Refinement procedure

Resolution	32.060 - 2.220
R-factor	0.1925
Rwork	0.191
R-free	0.23040
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1dnk
RMSD bond length	0.015
RMSD bond angle	1.738
Data reduction software	XDS
Data scaling software	XDS
Phasing software	PHASER
Refinement software	PHENIX (1.18.2_3874)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	32.070	2.250
High resolution limit [Å]	2.210	2.210
Rmerge	0.145	1.391
Rmeas	0.153	1.609
Number of reflections	50424	2368
<I/σ(I)>	8.5	0.9
Completeness [%]	94.9	90.3
Redundancy	9.1	3.9
CC(1/2)	0.998	0.491

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	8.5	283	19% PEG 8000, 250 mM Magnesium Chloride, 100 mM Tris pH 8.5