7KF1
Crystal structure of bH1 Fab variant (CDR H3 loop design 14_0130) in complex with VEGF
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | CLSI BEAMLINE 08ID-1 |
| Synchrotron site | CLSI |
| Beamline | 08ID-1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2018-06-17 |
| Detector | RAYONIX MX-300 |
| Wavelength(s) | 0.9795 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 194.167, 100.749, 153.509 |
| Unit cell angles | 90.00, 91.69, 90.00 |
Refinement procedure
| Resolution | 48.570 - 2.450 |
| R-factor | 0.1911 |
| Rwork | 0.189 |
| R-free | 0.23190 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3bdy |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.386 |
| Data reduction software | iMOSFLM |
| Data scaling software | SCALA (0.6.3) |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.8.0222) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 48.570 | 48.520 | 2.490 |
| High resolution limit [Å] | 2.450 | 13.420 | 2.450 |
| Rmerge | 0.087 | 0.046 | 0.628 |
| Rmeas | 0.104 | 0.055 | 0.757 |
| Rpim | 0.058 | 0.031 | 0.418 |
| Total number of observations | 2163 | 16611 | |
| Number of reflections | 107303 | 699 | 5259 |
| <I/σ(I)> | 8.9 | 16 | 2.1 |
| Completeness [%] | 98.8 | 97.5 | 98.2 |
| Redundancy | 3.2 | 3.1 | 3.2 |
| CC(1/2) | 0.995 | 0.996 | 0.571 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | MICROBATCH | 295 | 0.2 M Potassium chloride, 0.05 M HEPES pH 7.5, 35% (v/v) Pentaerythritol propoxylate |
