7K66

Structure of Blood Coagulation Factor VIII in Complex with an Anti-C1 Domain Pathogenic Antibody Inhibitor

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ALS BEAMLINE 5.0.2
Synchrotron site	ALS
Beamline	5.0.2
Temperature [K]	80
Detector technology	PIXEL
Collection date	2019-03-28
Detector	DECTRIS PILATUS3 S 6M
Wavelength(s)	1.0
Spacegroup name	P 41 21 2
Unit cell lengths	116.721, 116.721, 371.057
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	49.490 - 3.920
R-factor	0.2341
Rwork	0.226
R-free	0.32190
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	6mf0
Data reduction software	Adxv
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	PHENIX (1.18.2-3874)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	49.490	4.060
High resolution limit [Å]	3.920	3.920
Rmerge	0.100	0.932
Number of reflections	23431	2201
<I/σ(I)>	5.74
Completeness [%]	97.6	94.06
Redundancy	2	2
CC(1/2)	0.994	0.342

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7.7	293	17.5 %(w/v) PEG 1500, 50 mM HEPES (pH 7.7)