7FCP
Crystallographic structure of two neutralizing antibodies in complex with SARS-CoV-2 spike receptor-binding Domain (RBD)
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | NFPSS BEAMLINE BL19U1 |
Synchrotron site | NFPSS |
Beamline | BL19U1 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2021-01-08 |
Detector | DECTRIS PILATUS 6M |
Wavelength(s) | 0.97852 |
Spacegroup name | C 1 2 1 |
Unit cell lengths | 223.007, 80.414, 72.746 |
Unit cell angles | 90.00, 106.23, 90.00 |
Refinement procedure
Resolution | 50.000 - 2.400 |
R-factor | 0.1935 |
Rwork | 0.191 |
R-free | 0.24460 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 6xdg 7jmw |
RMSD bond length | 0.009 |
RMSD bond angle | 1.389 |
Data reduction software | HKL-2000 |
Data scaling software | SCALEPACK |
Phasing software | PHASER (2.6.1) |
Refinement software | REFMAC (5.8.0155) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 107.060 | 2.440 |
High resolution limit [Å] | 2.400 | 2.400 |
Rmerge | 0.080 | 0.719 |
Rmeas | 0.088 | 0.795 |
Number of reflections | 48529 | 2422 |
<I/σ(I)> | 22.4 | 2.2 |
Completeness [%] | 100.0 | 99.7 |
Redundancy | 6.3 | 5.4 |
CC(1/2) | 0.981 | 0.751 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7 | 291 | 0.05 M HEPES pH 7.0 20%(w/v) PEG3350 1%(w/v) Tryptone |