7F3O
Crystal structure of the GluA2o LBD in complex with glutamate and TAK-653
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ALS BEAMLINE 5.0.3 |
| Synchrotron site | ALS |
| Beamline | 5.0.3 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2012-03-07 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 0.9765 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 114.313, 163.508, 47.558 |
| Unit cell angles | 90.00, 89.99, 90.00 |
Refinement procedure
| Resolution | 40.000 - 1.440 |
| R-factor | 0.19486 |
| Rwork | 0.194 |
| R-free | 0.21381 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1ftj |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.815 |
| Data reduction software | HKL-2000 |
| Data scaling software | SCALEPACK |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.8.0267) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.460 |
| High resolution limit [Å] | 1.440 | 1.440 |
| Number of reflections | 304104 | 14115 |
| <I/σ(I)> | 21.2 | 1.6 |
| Completeness [%] | 97.1 | 91 |
| Redundancy | 4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 4.9 | 277 | 15% PEG 3350, 0.1M SODIUM ACETATE, 0.1M ZINC ACETATE, |
