7EZG

The structure of the human METTL6 enzyme in complex with SAH

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL19U1
Synchrotron site	SSRF
Beamline	BL19U1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2020-06-28
Detector	DECTRIS PILATUS3 6M
Wavelength(s)	0.979
Spacegroup name	P 2 3
Unit cell lengths	110.264, 110.264, 110.264
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	25.300 - 1.900
R-factor	0.1802
Rwork	0.179
R-free	0.20050
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	6c5b
RMSD bond length	0.008
RMSD bond angle	1.129
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	PHENIX (1.14_3260)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	1.970
High resolution limit [Å]	1.899	1.900
Rmerge	0.190	1.760
Number of reflections	35491	3525
<I/σ(I)>	28	2
Completeness [%]	100.0
Redundancy	38.5
CC(1/2)	0.998	0.750

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	9	298	PEGRX F7?25% PEG1500?0.1M BIS-Tris pH9.0?0.1M NaCl