7EVS

Crystal structure of hnRNP LL RRM2 in complex with SETD2

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL19U1
Synchrotron site	SSRF
Beamline	BL19U1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2021-01-01
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.979
Spacegroup name	P 1
Unit cell lengths	41.676, 41.685, 42.636
Unit cell angles	85.02, 89.57, 71.12

Refinement procedure

Resolution	30.210 - 1.600
R-factor	0.1789
Rwork	0.178
R-free	0.20360
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	7evr
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	MOLREP
Refinement software	PHENIX (1.19_4092)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	40.000	40.000	1.630
High resolution limit [Å]	1.600	4.340	1.600
Rmerge	0.063	0.043	0.314
Rmeas	0.075	0.051	0.373
Rpim	0.040	0.027	0.199
Total number of observations	118064
Number of reflections	34584	1763	1664
<I/σ(I)>	7.9
Completeness [%]	96.6	98.1	94.3
Redundancy	3.4	3.5	3.3
CC(1/2)		0.995	0.908

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		293	0.3M Ammonium Sulfate, 20% PEG 4000