7EOW
High-resolution structure of vWF A1 domain in complex with caplacizumab, the first nanobody-based medicine
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | PAL/PLS BEAMLINE 5C (4A) |
Synchrotron site | PAL/PLS |
Beamline | 5C (4A) |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2019-11-03 |
Detector | DECTRIS EIGER X 9M |
Wavelength(s) | 1 |
Spacegroup name | P 61 |
Unit cell lengths | 74.741, 74.741, 122.288 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 27.550 - 1.600 |
R-factor | 0.1765 |
Rwork | 0.175 |
R-free | 0.19840 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 5ggs |
RMSD bond length | 0.006 |
RMSD bond angle | 0.801 |
Data scaling software | HKL-2000 |
Phasing software | MOLREP |
Refinement software | PHENIX (1.15.2_3472) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 27.550 | 1.630 |
High resolution limit [Å] | 1.600 | 1.600 |
Rmeas | 0.064 | 0.541 |
Number of reflections | 48735 | 2549 |
<I/σ(I)> | 5.36 | 5.36 |
Completeness [%] | 95.4 | |
Redundancy | 5 | 10.5 |
CC(1/2) | 0.996 | 0.943 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 8 | 293.15 | 20 mM Tris-HCl pH 8 150 mM NaCl 10% glycerol 40% glycerol ethoxylate |