7D5O
C-Src in complex with TAS-120
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL17U |
| Synchrotron site | SSRF |
| Beamline | BL17U |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2020-05-31 |
| Detector | ADSC QUANTUM 1 |
| Wavelength(s) | 0.97915 |
| Spacegroup name | P 1 |
| Unit cell lengths | 41.969, 63.184, 73.137 |
| Unit cell angles | 100.64, 90.61, 89.82 |
Refinement procedure
| Resolution | 28.880 - 2.690 |
| R-factor | 0.1844 |
| Rwork | 0.178 |
| R-free | 0.23690 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4u5j |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHENIX |
| Refinement software | PHENIX (1.19.2_4158) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 28.880 | 2.781 |
| High resolution limit [Å] | 2.685 | 2.685 |
| Number of reflections | 19833 | 1589 |
| <I/σ(I)> | 9.33 | |
| Completeness [%] | 96.0 | |
| Redundancy | 3.3 | |
| CC(1/2) | 0.985 | 0.767 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.4 | 291 | 2% glycerol, 8% PEG 4000, 50mM sodium acetate, 10mM MgCl2 |
