7CKK

Structural complex of FTO bound with Dac51

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL17U
Synchrotron site	SSRF
Beamline	BL17U
Temperature [K]	100
Detector technology	CCD
Collection date	2018-10-12
Detector	ADSC QUANTUM 315r
Wavelength(s)	0.9735
Spacegroup name	H 3
Unit cell lengths	142.124, 142.124, 84.539
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	30.000 - 2.350
R-factor	0.2228
Rwork	0.221
R-free	0.26430
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4qkn
RMSD bond length	0.010
RMSD bond angle	1.318
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	REFMAC (5.8.0135)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	30.000	30.000	2.430
High resolution limit [Å]	2.350	5.060	2.350
Rmerge	0.057	0.028	1.162
Rmeas	0.060	0.030	1.232
Rpim	0.020	0.010	0.403
Total number of observations	260158
Number of reflections	26488	2657	2634
<I/σ(I)>	13.9
Completeness [%]	99.7	99.7	99.2
Redundancy	9.8	10	8.8
CC(1/2)		0.999	0.752

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		293	0.1 M sodium citrate (pH 5.8), 12 % (w/v) polyethylene glycol 3350, 8 % isopropanol