7CFO
Crystal structure of human RXRalpha ligand binding domain complexed with CBTF-EE.
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | PHOTON FACTORY BEAMLINE AR-NW12A |
| Synchrotron site | Photon Factory |
| Beamline | AR-NW12A |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2018-12-13 |
| Detector | DECTRIS PILATUS3 2M |
| Wavelength(s) | 1.0 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 50.757, 99.432, 93.598 |
| Unit cell angles | 90.00, 98.31, 90.00 |
Refinement procedure
| Resolution | 47.140 - 2.150 |
| R-factor | 0.22283 |
| Rwork | 0.221 |
| R-free | 0.26633 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 5zqu |
| RMSD bond length | 0.009 |
| RMSD bond angle | 2.001 |
| Data reduction software | HKL-2000 |
| Data scaling software | SCALEPACK |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.8.0238) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 47.140 | 2.230 |
| High resolution limit [Å] | 2.150 | 2.150 |
| Rmerge | 0.081 | 0.498 |
| Number of reflections | 48015 | 1815 |
| <I/σ(I)> | 16.2 | 1.7 |
| Completeness [%] | 95.6 | |
| Redundancy | 6.5 | |
| CC(1/2) | 0.996 | 0.860 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7 | 295 | 5% [v/v] tacsimateTM [pH 7.0], 0.1 M HEPES [pH 7.0], and 10% [w/v] PEGMME5000 |
