7C60
Crystal structure of Keap1 in complex with monoethyl fumarate (MEF)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2019-10-11 |
| Detector | RIGAKU HyPix-6000HE |
| Wavelength(s) | 1.54056 |
| Spacegroup name | P 61 |
| Unit cell lengths | 103.726, 103.726, 56.795 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 20.880 - 1.950 |
| R-factor | 0.2177 |
| Rwork | 0.214 |
| R-free | 0.28830 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1x2j |
| Data reduction software | CrysalisPro |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.18_3855) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 24.950 | 1.970 |
| High resolution limit [Å] | 1.600 | 1.600 |
| Rmerge | 0.140 | |
| Number of reflections | 26285 | 1377 |
| <I/σ(I)> | 8 | |
| Completeness [%] | 94.2 | |
| Redundancy | 8 | |
| CC(1/2) | 0.984 | 0.998 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 5 | 293 | Lithium Sulphate, PEG 8000, Sodium acetate, glycerol |
