7BXA

Crystal structure of PD-1 in complex with tislelizumab Fab

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PAL/PLS BEAMLINE 5C (4A)
Synchrotron site	PAL/PLS
Beamline	5C (4A)
Temperature [K]	100
Detector technology	PIXEL
Collection date	2019-05-08
Detector	DECTRIS EIGER X 9M
Wavelength(s)	1
Spacegroup name	P 21 21 21
Unit cell lengths	64.615, 73.081, 276.388
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	48.407 - 3.320
R-factor	0.2456
Rwork	0.243
R-free	0.29860
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4ky1
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHENIX
Refinement software	PHENIX (1.13_2998)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	3.410
High resolution limit [Å]	3.320	3.320
Number of reflections	19822	985
<I/σ(I)>	2
Completeness [%]	99.3
Redundancy	4.5
CC(1/2)	0.947	0.788

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		293	0.2 M ammonium sulfate, 30% w/v polyethylene glycol 4000