7BTS
Structure of human beta1 adrenergic receptor bound to epinephrine and nanobody 6B9
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SPRING-8 BEAMLINE BL32XU |
| Synchrotron site | SPring-8 |
| Beamline | BL32XU |
| Temperature [K] | 80 |
| Detector technology | PIXEL |
| Collection date | 2018-02-04 |
| Detector | DECTRIS EIGER X 9M |
| Wavelength(s) | 1.000 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 377.980, 66.220, 47.860 |
| Unit cell angles | 90.00, 93.88, 90.00 |
Refinement procedure
| Resolution | 19.980 - 3.130 |
| R-factor | 0.2374 |
| Rwork | 0.235 |
| R-free | 0.25850 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4lde |
| RMSD bond length | 0.015 |
| RMSD bond angle | 0.783 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | PHENIX |
| Refinement software | PHENIX (1.17.1_3660) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 3.200 |
| High resolution limit [Å] | 3.100 | 3.100 |
| Number of reflections | 20331 | 897 |
| <I/σ(I)> | 5.59 | 1.37 |
| Completeness [%] | 96.8 | 58.3 |
| Redundancy | 11.4 | 7.6 |
| CC(1/2) | 0.982 | 0.500 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | LIPIDIC CUBIC PHASE | 293 | 100 mM HEPES, pH 7.5, 80-180 mM Sodium Sulfate, 38-42% PEG400 or PEG300, 10mM epinephrine |
