7AVL
Crystal structure of SOS1 in complex with compound 4
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X06DA |
| Synchrotron site | SLS |
| Beamline | X06DA |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2015-05-08 |
| Detector | DECTRIS PILATUS 2M |
| Wavelength(s) | 1.000000 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 83.854, 39.824, 176.534 |
| Unit cell angles | 90.00, 90.04, 90.00 |
Refinement procedure
| Resolution | 83.850 - 1.718 |
| R-factor | 0.2506 |
| Rwork | 0.250 |
| R-free | 0.26930 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 7avi |
| RMSD bond length | 0.007 |
| RMSD bond angle | 0.800 |
| Data reduction software | autoPROC |
| Data scaling software | autoPROC (1.1.7) |
| Phasing software | PHASER |
| Refinement software | BUSTER (2.11.7) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 83.854 | 1.897 |
| High resolution limit [Å] | 1.718 | 1.718 |
| Rmerge | 0.055 | 1.621 |
| Number of reflections | 89761 | 4489 |
| <I/σ(I)> | 14.2 | 0.8 |
| Completeness [%] | 71.4 | 14 |
| Redundancy | 3.4 | 3.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 277 | 6-11 % PEG 8000, 60 mM Tris, 2mM DTT |
