7AL7
The Crystal Structure of Human IL-18 in Complex With Human IL-18 Binding Protein
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | PETRA III, EMBL c/o DESY BEAMLINE P14 (MX2) |
Synchrotron site | PETRA III, EMBL c/o DESY |
Beamline | P14 (MX2) |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2019-05-24 |
Detector | DECTRIS EIGER X 16M |
Wavelength(s) | 0.9762 |
Spacegroup name | C 1 2 1 |
Unit cell lengths | 109.815, 44.520, 60.276 |
Unit cell angles | 90.00, 99.86, 90.00 |
Refinement procedure
Resolution | 59.386 - 1.801 |
R-factor | 0.2111 |
Rwork | 0.209 |
R-free | 0.23150 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 3f62 |
Data reduction software | XDS (vMar 15, 2019) |
Data scaling software | XSCALE (vMar 15, 2019) |
Phasing software | PHASER (v2.8) |
Refinement software | PHENIX (1.14_3260) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 59.390 | 59.390 | 1.910 |
High resolution limit [Å] | 1.800 | 5.380 | 1.800 |
Rmerge | 0.048 | ||
Rmeas | 0.053 | 3.200 | 1.063 |
Rpim | 0.020 | ||
Number of reflections | 26507 | 1050 | 4179 |
<I/σ(I)> | 15.67 | 55.62 | 1.75 |
Completeness [%] | 98.3 | 99 | 97.6 |
Redundancy | 6.9 | ||
CC(1/2) | 0.999 | 0.999 | 0.948 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION | 6.5 | 293 | 0.2 M Calcium acetate hydrate, 0.1 M Sodium cacodylate trihydrate pH 6.5, 18% w/v Polyethylene glycol 8000 |