7ZKX
SRPK2 IN COMPLEX WITH INHIBITOR
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X10SA |
| Synchrotron site | SLS |
| Beamline | X10SA |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2019-01-16 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 0.99989 |
| Spacegroup name | P 31 2 1 |
| Unit cell lengths | 107.755, 107.755, 90.228 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 93.320 - 2.060 |
| R-factor | 0.1807 |
| Rwork | 0.179 |
| R-free | 0.20750 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2x7g |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.536 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.8.0155) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 93.320 | 2.310 |
| High resolution limit [Å] | 2.060 | 2.060 |
| Rmeas | 0.050 | 0.584 |
| Number of reflections | 36972 | 10714 |
| <I/σ(I)> | 15.51 | 2.53 |
| Completeness [%] | 97.9 | 98.8 |
| Redundancy | 2.9 | 2.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION | 293 | 5 mM HEPES pH 7.5, 150 mM NaCl, 20 mM DTT |
