7Z3J

Structure of crystallisable rat Phospholipase C gamma 1 in complex with inositol 1,4,5-trisphosphate

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE ID23-1
Synchrotron site	ESRF
Beamline	ID23-1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2021-11-07
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.88560
Spacegroup name	P 21 21 21
Unit cell lengths	72.760, 82.440, 230.110
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	49.290 - 2.000
R-factor	0.2254
Rwork	0.224
R-free	0.25640
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	6bpc
RMSD bond length	0.009
RMSD bond angle	1.006
Data reduction software	XDS
Data scaling software	XDS
Phasing software	MOLREP (11.7.03)
Refinement software	PHENIX (1.19.2_4158)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	115.100	2.050
High resolution limit [Å]	2.000	2.000
Rmeas	0.076
Number of reflections	93254	28825
<I/σ(I)>	10.83
Completeness [%]	98.8
Redundancy	13.5
CC(1/2)	0.999	0.234

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION	7	293	18.7 % PEG 3350, 0.1 M CBTP