7YXB

MHC-II dynamics are maintained in HLA-DR allotypes to ensure catalyzed peptide exchange

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	BESSY BEAMLINE 14.1
Synchrotron site	BESSY
Beamline	14.1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2017-03-07
Detector	DECTRIS PILATUS3 6M
Wavelength(s)	0.91841
Spacegroup name	C 2 2 21
Unit cell lengths	96.891, 111.485, 212.540
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	44.081 - 2.095
R-factor	0.1881
Rwork	0.187
R-free	0.22210
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4x5x
RMSD bond length	0.005
RMSD bond angle	0.741
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHASER
Refinement software	PHENIX (1.12_2829)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	44.081	2.220
High resolution limit [Å]	2.090	2.090
Rmeas	0.016	0.151
Number of reflections	67120	10573
<I/σ(I)>	9.69	1.28
Completeness [%]	99.0
Redundancy	4.56
CC(1/2)	0.996	0.491

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		293	20% PEG 3350, 0.2M Ammoniumcitrate