7UM4
Crystal structure of inactive 5-HT5AR in complex with AS2674723
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 23-ID-D |
Synchrotron site | APS |
Beamline | 23-ID-D |
Temperature [K] | 80 |
Detector technology | PIXEL |
Collection date | 2019-08-13 |
Detector | DECTRIS PILATUS3 6M |
Wavelength(s) | 1.033 |
Spacegroup name | P 21 21 2 |
Unit cell lengths | 135.289, 42.460, 94.787 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 50.000 | 50.000 | 2.900 |
High resolution limit [Å] | 2.800 | 6.030 | 2.800 |
Rmerge | 0.307 | 0.205 | 1.024 |
Rmeas | 0.332 | 0.221 | 1.185 |
Rpim | 0.122 | 0.079 | 0.581 |
Number of reflections | 13732 | 1488 | 1285 |
<I/σ(I)> | 5.8 | ||
Completeness [%] | 99.2 | 98 | 97.2 |
Redundancy | 6.6 | 7.4 | 3.6 |
CC(1/2) | 0.960 | 0.554 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | LIPIDIC CUBIC PHASE | 293 | 0.1 M Sodium chloride, 0.1 M Lithium sulfate, 0.1 M DL-Malic acid pH 5.9, 30 % v/v PEG 400 |