7U0L

Crystal structure of the CCoV-HuPn-2018 RBD (domain B) in complex with canine APN

This is a non-PDB format compatible entry.

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ALS BEAMLINE 5.0.2
Synchrotron site	ALS
Beamline	5.0.2
Temperature [K]	80
Detector technology	PIXEL
Collection date	2021-12-16
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.988
Spacegroup name	P 21 2 21
Unit cell lengths	101.431, 107.329, 239.229
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	48.960 - 3.300
R-factor	0.2167
Rwork	0.215
R-free	0.24010
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4f5c
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	PHENIX
Refinement software	PHENIX (1.19.2_4158)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	49.610	3.400
High resolution limit [Å]	3.300	3.300
Rmerge	0.065
Number of reflections	40080	4470
<I/σ(I)>	6.5
Completeness [%]	100.0
Redundancy	13.4
CC(1/2)		0.998

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		295	0.1 M Na2HPO4-citric acid pH 4.2, 1,6-,0.4 M Na2HPO4/K2HPO4