7TD5

Structure of human PRC2-EZH1 containing phosphorylated SUZ12

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 19-ID
Synchrotron site	APS
Beamline	19-ID
Temperature [K]	93
Detector technology	PIXEL
Collection date	2017-12-01
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.979
Spacegroup name	C 1 2 1
Unit cell lengths	231.870, 64.428, 254.496
Unit cell angles	90.00, 109.89, 90.00

Refinement procedure

Resolution	42.520 - 2.994
Rwork	0.196
R-free	0.22990
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	5wg6
RMSD bond length	0.008
RMSD bond angle	1.000
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	BUSTER (2.10.4 (16-JUL-2021))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	3.050
High resolution limit [Å]	2.994	2.994
Number of reflections	72155	3560
<I/σ(I)>	19.15
Completeness [%]	85.7
Redundancy	6.6
CC(1/2)	0.995	0.602

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		289	10% PEG3350, 100 mM (NH4)2SO4, 50mM HEPES 6.8