7SOL

Crystal Structures of the bispecific ubiquitin/FAT10 activating enzyme, Uba6

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 24-ID-E
Synchrotron site	APS
Beamline	24-ID-E
Temperature [K]	108
Detector technology	CCD
Collection date	2019-10-30
Detector	ADSC QUANTUM 315
Wavelength(s)	0.979
Spacegroup name	C 1 2 1
Unit cell lengths	248.572, 101.317, 122.921
Unit cell angles	90.00, 118.00, 90.00

Refinement procedure

Resolution	45.905 - 2.250
R-factor	0.166435680207
Rwork	0.166
R-free	0.20570
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	6dc6
RMSD bond length	0.004
RMSD bond angle	0.718
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	PHENIX (1.10.1_2155)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.290
High resolution limit [Å]	2.250	2.250
Number of reflections	125930	5797
<I/σ(I)>	12.1
Completeness [%]	98.0
Redundancy	4.75
CC(1/2)	0.996	0.777

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		291	20 % PEG 3350, 0.2 M NaF