7S7J

Structure of Human SPASTIN-IST1 complex.

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRL BEAMLINE BL9-1
Synchrotron site	SSRL
Beamline	BL9-1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2016-03-13
Detector	CS-PAD CXI-1
Wavelength(s)	0.97946
Spacegroup name	I 41
Unit cell lengths	79.614, 79.614, 36.331
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	33.050 - 1.150
R-factor	0.1491
Rwork	0.149
R-free	0.15720
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	3eab
RMSD bond length	0.007
RMSD bond angle	0.907
Data reduction software	XDS
Data scaling software	XDS
Phasing software	PHENIX (1.17.1_3660)
Refinement software	PHENIX (1.17.1_3660)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	33.050	1.170
High resolution limit [Å]	1.150	1.150
Rmerge	0.103	1.647
Rmeas	0.107
Rpim	0.029
Number of reflections	74704	1425
<I/σ(I)>	16.4	4
Completeness [%]	94.2	72.2
Redundancy	13.6	12.3
CC(1/2)	0.998	0.865

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	6.5	278	40% v/v PEG 300, 100 mM Sodium cacodylate / Hydrochloric acid pH=6.5, 200 mM Calcium Acetate