7RM4

Neoantigen p53R175H-specific TCR 6-11 binds to p53R175H-HLA-A2

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 23-ID-D
Synchrotron site	APS
Beamline	23-ID-D
Temperature [K]	100
Detector technology	PIXEL
Collection date	2020-07-22
Detector	DECTRIS PILATUS3 S 6M
Wavelength(s)	1.0
Spacegroup name	P 1 21 1
Unit cell lengths	127.226, 55.144, 304.877
Unit cell angles	90.00, 98.74, 90.00

Refinement procedure

Resolution	49.319 - 3.330
R-factor	0.2397
Rwork	0.237
R-free	0.29490
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	5wkf 6ovn 6vr5
Data reduction software	HKL-3000
Data scaling software	HKL-3000
Phasing software	PHENIX
Refinement software	PHENIX (1.16_3549)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	49.319	3.450
High resolution limit [Å]	3.330	3.330
Number of reflections	60837	4526
<I/σ(I)>	10.3
Completeness [%]	92.6
Redundancy	4.8
CC(1/2)	0.992	0.737

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	8.5	293	20% (w/v) PEG 3350, 0.1 M Bis-Tris propane (pH 8.5), and 0.2 M Potassium thiocyanate