7RCH

Crystal structure of NS1-ED of Vietnam influenza A virus in complex with the p85-beta-iSH2 domain of human PI3K

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU MICROMAX-007 HF
Temperature [K]	120
Detector technology	IMAGE PLATE
Collection date	2021-01-15
Detector	RIGAKU RAXIS IV++
Wavelength(s)	1.5418
Spacegroup name	P 1 21 1
Unit cell lengths	61.440, 92.840, 67.120
Unit cell angles	90.00, 105.03, 90.00

Refinement procedure

Resolution	50.000 - 3.100
R-factor	0.2444
Rwork	0.243
R-free	0.27620
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	6ox7
RMSD bond length	0.002
RMSD bond angle	0.447
Data reduction software	MOSFLM
Data scaling software	HKL-2000
Phasing software	PHENIX
Refinement software	PHENIX (1.19.1_4122)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	64.820	3.310
High resolution limit [Å]	3.100	3.100
Number of reflections	13030	2318
<I/σ(I)>	4
Completeness [%]	99.0
Redundancy	3.52
CC(1/2)	0.975	0.614

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	SMALL TUBES		278	20 mM sodium phosphate, pH 7.0, 80 mM sodium chloride