7R5L

Crystal structure of YTHDF2 with compound YLI_DC1_015

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SLS BEAMLINE X06DA
Synchrotron site	SLS
Beamline	X06DA
Temperature [K]	100
Detector technology	PIXEL
Collection date	2021-08-28
Detector	DECTRIS PILATUS 2M-F
Wavelength(s)	1
Spacegroup name	P 65 2 2
Unit cell lengths	76.240, 76.240, 115.840
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	38.120 - 1.700
R-factor	0.2045
Rwork	0.204
R-free	0.22360
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4rdn
RMSD bond length	0.006
RMSD bond angle	0.829
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHASER
Refinement software	PHENIX (1.20_4459)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	38.120	1.800
High resolution limit [Å]	1.700	1.700
Number of reflections	22678	3523
<I/σ(I)>	26.77
Completeness [%]	99.8
Redundancy	19.68
CC(1/2)	1.000	0.901

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		277.15	1.95 M Ammonium sulfate, 0.1 M Sodium citrate tribasic pH 5.0