7Q15
Crystal structure of FcRn and beta-2-microglobulin in complex with IgG1-Fc-MST-HN (efgartigimod)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SOLEIL BEAMLINE PROXIMA 2 |
| Synchrotron site | SOLEIL |
| Beamline | PROXIMA 2 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2019-03-22 |
| Detector | DECTRIS EIGER X 9M |
| Wavelength(s) | 0.89 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 86.429, 53.107, 195.745 |
| Unit cell angles | 90.00, 92.18, 90.00 |
Refinement procedure
| Resolution | 48.900 - 3.301 |
| R-factor | 0.2077 |
| Rwork | 0.206 |
| R-free | 0.23130 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1n0u |
| RMSD bond length | 0.005 |
| RMSD bond angle | 0.810 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | PHASER |
| Refinement software | BUSTER (2.10.3) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 48.901 | 3.449 |
| High resolution limit [Å] | 3.146 | 3.146 |
| Rmeas | 0.380 | 0.016 |
| Number of reflections | 21510 | 1073 |
| <I/σ(I)> | 4.2 | 1.1 |
| Completeness [%] | 90.4 | |
| Redundancy | 3.1 | |
| CC(1/2) | 0.942 | 0.265 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7 | 294 | 0.1 M Sodium Cacodylate pH 7 |
