7OM4

Nanobody EgB4 bound to the full extracellular EGFR-EGF complex

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	DIAMOND BEAMLINE I24
Synchrotron site	Diamond
Beamline	I24
Temperature [K]	100
Detector technology	PIXEL
Collection date	2019-01-31
Detector	DECTRIS PILATUS3 6M
Wavelength(s)	0.9686
Spacegroup name	P 61 2 2
Unit cell lengths	307.610, 307.610, 135.138
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	153.810 - 6.050
R-factor	0.2968
Rwork	0.296
R-free	0.32680
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	3njp
Data reduction software	XDS
Data scaling software	STARANISO
Phasing software	PHASER
Refinement software	PHENIX (1.19.1_4122)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	153.810	7.150
High resolution limit [Å]	6.050	6.050
Rmerge	0.187	1.995
Rmeas	0.199	2.099
Rpim	0.065	0.642
Number of reflections	6321	632
<I/σ(I)>	7.8	1.6
Completeness [%]	91.8
Redundancy	9.6
CC(1/2)	0.996	0.548

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		293	0.1 M LiSO4, 0.1 M glycine pH 10.5, 1.1 M sodium dihydrogen phosphate and 0.72 M dipotassium hydrogen phosphate