7LW9

Human Exonuclease 5 crystal structure in complex with ssDNA, Sm, and Na

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ALS BEAMLINE 8.3.1
Synchrotron site	ALS
Beamline	8.3.1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2019-04-04
Detector	DECTRIS PILATUS3 6M
Wavelength(s)	1.1161
Spacegroup name	P 2 21 21
Unit cell lengths	51.073, 83.664, 96.150
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	45.105 - 2.710
R-factor	0.1967
Rwork	0.194
R-free	0.25320
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	7lw7
RMSD bond length	0.003
RMSD bond angle	0.590
Data reduction software	XDS
Data scaling software	Aimless (0.7.4)
Phasing software	PHASER (2.7.16)
Refinement software	PHENIX (1.16_3549)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	45.105	45.100	2.840
High resolution limit [Å]	2.710	8.990	2.710
Rmerge	0.317	0.057	3.070
Rmeas	0.329	0.060	3.194
Rpim	0.090	0.018	0.870
Total number of observations		4028	19957
Number of reflections	11691	380	1516
<I/σ(I)>	8.9	35.1	1.1
Completeness [%]	99.7	99.2	99.7
Redundancy	13	10.6	13.2
CC(1/2)	0.995	0.998	0.394

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		293	20 % PEG 3350 and 0.2 M ammonium acetate