7LPS

Crystal structure of DDB1-CRBN-ALV1 complex bound to Helios (IKZF2 ZF2)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 24-ID-C
Synchrotron site	APS
Beamline	24-ID-C
Temperature [K]	100
Detector technology	PIXEL
Collection date	2019-07-12
Detector	DECTRIS PILATUS 6M-F
Wavelength(s)	0.97920
Spacegroup name	P 1 21 1
Unit cell lengths	151.800, 117.292, 196.713
Unit cell angles	90.00, 97.30, 90.00

Refinement procedure

Resolution	150.570 - 3.780
R-factor	0.2895
Rwork	0.289
R-free	0.30310
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	5fqd
RMSD bond length	0.004
RMSD bond angle	0.700
Data reduction software	XDS (VERSION Mar 15, 2019 BUILT=20190315)
Data scaling software	Aimless (0.5.32)
Phasing software	PHASER
Refinement software	BUSTER (2.10.4 (11-DEC-2020))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	150.570	3.860
High resolution limit [Å]	3.780	3.780
Rmerge	0.367	1.813
Rpim	0.286	1.439
Number of reflections	67972	4033
<I/σ(I)>	3.2	0.7
Completeness [%]	98.2	87.6
Redundancy	4.6	4.5
CC(1/2)	0.988	0.280

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	8.57	293	30% (w/v) PEG3350, 0.1 M Tris pH 8.57