7KX0

Crystal structure of the CD27:CD70 co-stimulatory complex

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	CLSI BEAMLINE 08ID-1
Synchrotron site	CLSI
Beamline	08ID-1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2020-01-23
Detector	DECTRIS PILATUS3 6M
Wavelength(s)	0.9795
Spacegroup name	I 2 2 2
Unit cell lengths	109.530, 113.630, 163.510
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	36.720 - 2.690
R-factor	0.202
Rwork	0.200
R-free	0.23600
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	2re9 5tl5
RMSD bond length	0.008
RMSD bond angle	1.090
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	MOLREP
Refinement software	BUSTER (2.11.7)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	93.310	2.980
High resolution limit [Å]	2.690	2.690
Rmerge	0.105	1.180
Rpim	0.044	0.479
Number of reflections	18602	931
<I/σ(I)>	12	1.7
Completeness [%]	64.9	12.4
Redundancy	6.7	7
CC(1/2)	0.998	0.727

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	8	291	100 mM Tris hydrochloride pH 8 40 % 2-methyl-2,4-pentanediol