7KPG

Blocking Fab 25 anti-SIRP-alpha antibody in complex with SIRP-alpha Variant 1

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	RIGAKU FR-E SUPERBRIGHT
Temperature [K]	100
Detector technology	CCD
Collection date	2019-03-26
Detector	RIGAKU SATURN 92
Wavelength(s)	1.54
Spacegroup name	P 61 2 2
Unit cell lengths	189.160, 189.160, 78.400
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	61.920 - 2.270
R-factor	0.2367
Rwork	0.234
R-free	0.28190
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	2uv3
RMSD bond length	0.010
RMSD bond angle	1.481
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	REFMAC (5.8.0230)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	61.920	2.320
High resolution limit [Å]	2.270	2.270
Number of reflections	38395	2803
<I/σ(I)>	10.9
Completeness [%]	99.6
Redundancy	13.5
CC(1/2)	1.000	1.000

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION		277	28% w/v PEG 8000, 0.1 M citric acid pH 3.5