7KIU
Structure of recombinant human DNase1L3 in complex with Mg2+
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRL BEAMLINE BL14-1 |
| Synchrotron site | SSRL |
| Beamline | BL14-1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2019-12-15 |
| Detector | RAYONIX MX325HE |
| Wavelength(s) | 1.12709 |
| Spacegroup name | P 1 |
| Unit cell lengths | 52.313, 72.671, 73.169 |
| Unit cell angles | 85.39, 80.56, 88.39 |
Refinement procedure
| Resolution | 32.060 - 2.220 |
| R-factor | 0.1925 |
| Rwork | 0.191 |
| R-free | 0.23040 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1dnk |
| RMSD bond length | 0.015 |
| RMSD bond angle | 1.738 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.18.2_3874) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 32.070 | 2.250 |
| High resolution limit [Å] | 2.210 | 2.210 |
| Rmerge | 0.145 | 1.391 |
| Rmeas | 0.153 | 1.609 |
| Number of reflections | 50424 | 2368 |
| <I/σ(I)> | 8.5 | 0.9 |
| Completeness [%] | 94.9 | 90.3 |
| Redundancy | 9.1 | 3.9 |
| CC(1/2) | 0.998 | 0.491 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8.5 | 283 | 19% PEG 8000, 250 mM Magnesium Chloride, 100 mM Tris pH 8.5 |
