7JKL
Human PrimPol misinserting dATP opposite the 8-oxoguanine lesion (3'-end base of the primer strand is 2',3'-dideoxy-terminated).
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 24-ID-C |
| Synchrotron site | APS |
| Beamline | 24-ID-C |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2016-07-19 |
| Detector | DECTRIS PILATUS3 S 6M |
| Wavelength(s) | 0.97921 |
| Spacegroup name | P 1 |
| Unit cell lengths | 51.525, 65.641, 74.307 |
| Unit cell angles | 69.44, 83.43, 87.74 |
Refinement procedure
| Resolution | 69.170 - 2.384 |
| R-factor | 0.2224 |
| Rwork | 0.220 |
| R-free | 0.27700 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 5l2x |
| RMSD bond length | 0.005 |
| RMSD bond angle | 0.497 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.11.1_2575) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 69.170 | 2.460 |
| High resolution limit [Å] | 2.370 | 2.370 |
| Rmerge | 0.108 | |
| Number of reflections | 33664 | 2495 |
| <I/σ(I)> | 7 | |
| Completeness [%] | 91.2 | 64 |
| Redundancy | 3.5 | 2.9 |
| CC(1/2) | 0.993 | 0.449 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 293 | 225- 250 mM CaCl2, 16 -19% PEG 3350 |
