7FCP

Crystallographic structure of two neutralizing antibodies in complex with SARS-CoV-2 spike receptor-binding Domain (RBD)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	NFPSS BEAMLINE BL19U1
Synchrotron site	NFPSS
Beamline	BL19U1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2021-01-08
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.97852
Spacegroup name	C 1 2 1
Unit cell lengths	223.007, 80.414, 72.746
Unit cell angles	90.00, 106.23, 90.00

Refinement procedure

Resolution	50.000 - 2.400
R-factor	0.1935
Rwork	0.191
R-free	0.24460
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	6xdg 7jmw
RMSD bond length	0.009
RMSD bond angle	1.389
Data reduction software	HKL-2000
Data scaling software	SCALEPACK
Phasing software	PHASER (2.6.1)
Refinement software	REFMAC (5.8.0155)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	107.060	2.440
High resolution limit [Å]	2.400	2.400
Rmerge	0.080	0.719
Rmeas	0.088	0.795
Number of reflections	48529	2422
<I/σ(I)>	22.4	2.2
Completeness [%]	100.0	99.7
Redundancy	6.3	5.4
CC(1/2)	0.981	0.751

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7	291	0.05 M HEPES pH 7.0 20%(w/v) PEG3350 1%(w/v) Tryptone