7C60
Crystal structure of Keap1 in complex with monoethyl fumarate (MEF)
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | ROTATING ANODE |
Source details | RIGAKU |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2019-10-11 |
Detector | RIGAKU HyPix-6000HE |
Wavelength(s) | 1.54056 |
Spacegroup name | P 61 |
Unit cell lengths | 103.726, 103.726, 56.795 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 20.880 - 1.950 |
R-factor | 0.2177 |
Rwork | 0.214 |
R-free | 0.28830 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1x2j |
Data reduction software | CrysalisPro |
Data scaling software | SCALA |
Phasing software | PHASER |
Refinement software | PHENIX (1.18_3855) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 24.950 | 1.970 |
High resolution limit [Å] | 1.600 | 1.600 |
Rmerge | 0.140 | |
Number of reflections | 26285 | 1377 |
<I/σ(I)> | 8 | |
Completeness [%] | 94.2 | |
Redundancy | 8 | |
CC(1/2) | 0.984 | 0.998 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 5 | 293 | Lithium Sulphate, PEG 8000, Sodium acetate, glycerol |