7BQV

Cereblon in complex with SALL4 and (S)-5-hydroxythalidomide

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PHOTON FACTORY BEAMLINE AR-NE3A
Synchrotron site	Photon Factory
Beamline	AR-NE3A
Temperature [K]	95
Detector technology	PIXEL
Collection date	2019-10-31
Detector	DECTRIS PILATUS 2M-F
Wavelength(s)	1.0000
Spacegroup name	C 2 2 21
Unit cell lengths	83.621, 93.895, 43.682
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	35.794 - 1.800
R-factor	0.1983
Rwork	0.197
R-free	0.22340
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	7bqu
RMSD bond length	0.006
RMSD bond angle	0.858
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	PHASER (Ver 1.15.1_3469)
Refinement software	PHENIX (Ver 1.15.1_3469)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	46.950	1.840
High resolution limit [Å]	1.800	1.800
Rmeas	0.088	1.375
Rpim	0.024	0.373
Number of reflections	16362	957
<I/σ(I)>	21	2.3
Completeness [%]	100.0	100
Redundancy	13.1	13.3
CC(1/2)	1.000	0.832

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		293	22% PEG 4000, 0.1 M MES (pH 6.0), 0.2 M lithium sulfate