7BI2

PI3KC2aDeltaN and DeltaC-C2

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	BESSY BEAMLINE 14.1
Synchrotron site	BESSY
Beamline	14.1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2019-05-12
Detector	DECTRIS PILATUS3 6M
Wavelength(s)	0.9184
Spacegroup name	P 21 21 21
Unit cell lengths	82.557, 115.887, 144.365
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	49.200 - 3.250
R-factor	0.2687
Rwork	0.266
R-free	0.31180
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1e8x 2iwl
RMSD bond length	0.003
RMSD bond angle	1.166
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHASER
Refinement software	REFMAC (5.8.0258)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	49.200	3.450
High resolution limit [Å]	3.250	3.250
Rmeas	0.152	2.294
Number of reflections	22257	3455
<I/σ(I)>	9.73	0.84
Completeness [%]	99.1	97.1
Redundancy	4.9	4.9
CC(1/2)	0.998	0.443

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7.5	298	0.1MTris, 8% PEG20000, 10% ethylene glycol, 10% formamide