7B0I

Structure of a minimal SF3B core in complex with spliceostatin A (form II)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SLS BEAMLINE X10SA
Synchrotron site	SLS
Beamline	X10SA
Temperature [K]	100
Detector technology	PIXEL
Collection date	2019-03-18
Detector	DECTRIS PILATUS 6M-F
Wavelength(s)	1.0
Spacegroup name	P 32 2 1
Unit cell lengths	108.503, 108.503, 358.938
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	45.010 - 3.000
R-factor	0.217
Rwork	0.215
R-free	0.24680
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	6en4 5ife
RMSD bond length	0.002
RMSD bond angle	0.498
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	PHENIX (1.19_4092)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	49.410	3.040
High resolution limit [Å]	2.950	2.950
Rmerge	0.116	6.113
Rmeas	0.119	6.275
Rpim	0.027	1.403
Number of reflections	52617	4460
<I/σ(I)>	17.6	0.6
Completeness [%]	99.9	99.7
Redundancy	19.8	19.6
CC(1/2)	1.000	0.402

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7.17	277.15	0.1 M HEPES pH=7.17, 0.2 M MgCl2, 25% (v/v) PEG-400