7B0I
Structure of a minimal SF3B core in complex with spliceostatin A (form II)
Entity
| Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
| 1 | A (A) | Splicing factor 3B subunit 3,Splicing factor 3B subunit 3,Splicing factor 3B subunit 3 | polymer | 899 | 100722.1 | 1 | UniProt (Q15393) Pfam (PF10433) Pfam (PF03178) | Homo sapiens (Human) | Pre-mRNA-splicing factor SF3b 130 kDa subunit,SF3b130,STAF130,Spliceosome-associated protein 130,SAP 130,Pre-mRNA-splicing factor SF3b 130 kDa subunit,SF3b130,STAF130,Spliceosome-associated protein 130,SAP 130,Pre-mRNA-splicing factor SF3b 130 kDa subunit,SF3b130,STAF130,Spliceosome-associated protein 130,SAP 130 |
| 2 | B (B) | Splicing factor 3B subunit 5 | polymer | 86 | 10149.4 | 1 | UniProt (Q9BWJ5) Pfam (PF07189) | Homo sapiens (Human) | SF3b5,Pre-mRNA-splicing factor SF3b 10 kDa subunit |
| 3 | C (C) | Splicing factor 3B subunit 1 | polymer | 852 | 96615.7 | 1 | UniProt (O75533) Pfam (PF22646) | Homo sapiens (Human) | Pre-mRNA-splicing factor SF3b 155 kDa subunit,SF3b155,Spliceosome-associated protein 155,SAP 155 |
| 4 | D (D) | PHD finger-like domain-containing protein 5A | polymer | 108 | 11670.5 | 1 | UniProt (Q7RTV0) Pfam (PF03660) | Homo sapiens (Human) | PHD finger-like domain protein 5A,Splicing factor 3B-associated 14 kDa protein,SF3b14b |
| 5 | E (D) | spliceostatin A (form II) | non-polymer | 523.7 | 1 | Chemie (SJT) | |||
| 6 | F, G, H (D) | ZINC ION | non-polymer | 65.4 | 3 | Chemie (ZN) | |||
| 7 | I (D) | water | water | 18.0 | 1 | Chemie (HOH) |
Sequence modifications
A: 1 - 760 (UniProt: Q15393)
A: 768 - 1067 (UniProt: Q15393)
A: 1068 - 1199 (UniProt: Q15393)
D: 1 - 98 (UniProt: Q7RTV0)
| PDB | External Database | Details |
|---|---|---|
| Gly -9 | - | expression tag |
| Ala -8 | - | expression tag |
| Glu -7 | - | expression tag |
| Phe -6 | - | expression tag |
| Lys -5 | - | expression tag |
| Gly -4 | - | expression tag |
| Leu -3 | - | expression tag |
| Arg -2 | - | expression tag |
| Arg -1 | - | expression tag |
| His 0 | - | expression tag |
| PDB | External Database | Details |
|---|---|---|
| Gly 761 | - | linker |
| Gly 762 | - | linker |
| Asn 763 | - | linker |
| Gly 764 | - | linker |
| Asn 765 | - | linker |
| Ser 766 | - | linker |
| Gly 767 | - | linker |
| PDB | External Database | Details |
|---|---|---|
| Asp 1200 | - | expression tag |
| Tyr 1201 | - | expression tag |
| Lys 1202 | - | expression tag |
| Asp 1203 | - | expression tag |
| Asp 1204 | - | expression tag |
| Asp 1205 | - | expression tag |
| Asp 1206 | - | expression tag |
| Lys 1207 | - | expression tag |
| PDB | External Database | Details |
|---|---|---|
| Gly -9 | - | expression tag |
| Pro -8 | - | expression tag |
| Leu -7 | - | expression tag |
| Gly -6 | - | expression tag |
| Ser -5 | - | expression tag |
| Pro -4 | - | expression tag |
| Gly -3 | - | expression tag |
| Ser -2 | - | expression tag |
| Arg -1 | - | expression tag |
| Ala 0 | - | expression tag |
Sequence viewer
Contents of the asymmetric unit
| Polymers | Number of chains | 4 |
| Total formula weight | 219157.7 | |
| Non-Polymers* | Number of molecules | 4 |
| Total formula weight | 719.9 | |
| All* | Total formula weight | 219877.6 |
