7AUD

Structure of an engineered helicase domain construct for human Bloom syndrome protein (BLM)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	DIAMOND BEAMLINE I03
Synchrotron site	Diamond
Beamline	I03
Temperature [K]	100
Detector technology	PIXEL
Collection date	2017-01-30
Detector	DECTRIS PILATUS3 6M
Wavelength(s)	0.97623
Spacegroup name	P 1
Unit cell lengths	84.916, 111.709, 132.344
Unit cell angles	72.64, 80.18, 79.22

Refinement procedure

Resolution	47.280 - 2.960
R-factor	0.2311
Rwork	0.229
R-free	0.26900
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4o3m
Data reduction software	Aimless
Data scaling software	XDS
Phasing software	PHASER
Refinement software	PHENIX (1.18.2_3874)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	47.280	3.070
High resolution limit [Å]	2.960	2.970
Number of reflections	91662	8892
<I/σ(I)>	7.8	1.4
Completeness [%]	98.0	94.5
Redundancy	2.6
CC(1/2)	0.990	0.570

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6.5	277.15	Morpheus HT-96, Condition C9, Molecular Dimensions. 0.09 M NPS, 0.1M Buffer System, 30% Precipitant Mix 1 NPS = 0.3 M sodium nitrate, 0.3 M sodium phosphate dibasic, 0.3 M ammonium sulphate Buffer System 1 = 1.0 M imidazole, MES monohydrate (acid) pH 6.5 60% Precipitant Mix 1 = 40% v/v PEG 500 MME, 20% w/v PEG 20000