7AL7

The Crystal Structure of Human IL-18 in Complex With Human IL-18 Binding Protein

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PETRA III, EMBL c/o DESY BEAMLINE P14 (MX2)
Synchrotron site	PETRA III, EMBL c/o DESY
Beamline	P14 (MX2)
Temperature [K]	100
Detector technology	PIXEL
Collection date	2019-05-24
Detector	DECTRIS EIGER X 16M
Wavelength(s)	0.9762
Spacegroup name	C 1 2 1
Unit cell lengths	109.815, 44.520, 60.276
Unit cell angles	90.00, 99.86, 90.00

Refinement procedure

Resolution	59.386 - 1.801
R-factor	0.2111
Rwork	0.209
R-free	0.23150
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	3f62
Data reduction software	XDS (vMar 15, 2019)
Data scaling software	XSCALE (vMar 15, 2019)
Phasing software	PHASER (v2.8)
Refinement software	PHENIX (1.14_3260)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	59.390	59.390	1.910
High resolution limit [Å]	1.800	5.380	1.800
Rmerge	0.048
Rmeas	0.053	3.200	1.063
Rpim	0.020
Number of reflections	26507	1050	4179
<I/σ(I)>	15.67	55.62	1.75
Completeness [%]	98.3	99	97.6
Redundancy	6.9
CC(1/2)	0.999	0.999	0.948

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION	6.5	293	0.2 M Calcium acetate hydrate, 0.1 M Sodium cacodylate trihydrate pH 6.5, 18% w/v Polyethylene glycol 8000