7AHV

Anti-FIXa Fab of mim8 in complex with human FIXa

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SLS BEAMLINE X10SA
Synchrotron site	SLS
Beamline	X10SA
Temperature [K]	100
Detector technology	PIXEL
Collection date	2019-04-14
Detector	DECTRIS PILATUS3 6M
Wavelength(s)	1.0000
Spacegroup name	P 41 2 2
Unit cell lengths	97.030, 97.030, 254.510
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	48.520 - 3.110
R-factor	0.2283
Rwork	0.227
R-free	0.28350
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4pub 3kcg
RMSD bond length	0.012
RMSD bond angle	1.670
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	MOLREP
Refinement software	PHENIX (dev_3965)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	48.520	3.216
High resolution limit [Å]	3.105	3.105
Rmerge	0.692	2.568
Rmeas	0.721	2.672
Rpim	0.199	0.730
Number of reflections	22811	28675
<I/σ(I)>	4.3	0.95
Completeness [%]	99.8	99.19
Redundancy	13	13
CC(1/2)	0.957	0.476

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7.5	291	2 M ammonium sulphate, 0.1 M Hepes, pH 7.5